Teaching Sets

These sets group stocks from the collection for undergraduate genetics teaching. Each set has been used in published laboratory projects, linked below. To order a set or individual stocks from it, contact us at rcbc@rcbcseeds.com. All stocks are also listed in the full stock list (PDF).

Set A: Anthocyanin inheritance and selection

Parental, F1 and F2 populations for studying a single-gene trait (anthocyaninless) together with quantitative variation in anthocyanin intensity, including F2 lines selected for high and low anthocyanin.

  • 1-37: P1 parent. Anthocyaninless, hairy, male sterile
  • 1-155: F1. (Anthocyaninless/+), Pan(3/8), (hairless/hairy)
  • 1-157: F2, unselected population
  • 1-158: F2, selected from 1-155 for high anthocyanin
  • 1-159: F2, selected from 1-155 for low anthocyanin

Reference: Batzli, J.M., Smith, A.R., Williams, P.H., McGee, S.A., Dósa, K. and Pfammatter, J. (2014). Beyond Punnett squares: student word association and explanations of phenotypic variation through an integrative quantitative genetics unit investigating anthocyanin inheritance and expression in Brassica rapa Fast Plants. CBE-Life Sciences Education 13: 410-424. doi.org/10.1187/cbe.13-12-0232

Set B: Marker-defined strains (Wendell set)

Three strains, each fixed for different alleles at several DNA markers and at the anthocyaninless and yellow-green genes, plus the three F1 hybrids among them. Used for paternity-testing and linkage-mapping lab projects. Developed by Douglas L. Wendell, Oakland University.

  • 1-163: DWRCBr53 (improved line). Anthocyaninless
  • 1-164: DWRCBr76. Strong anthocyanin expression
  • 1-165: DWRCBr91. Anthocyaninless, yellow-green
  • 1-166: F1, 1-163 × 1-164
  • 1-167: F1, 1-163 × 1-165
  • 1-168: F1, 1-165 × 1-164

References: Slankster, E.E., Chase, J.M., Jones, L.A. and Wendell, D.L. (2012). DNA-based genetic markers for rapid cycling Brassica rapa (Fast Plants type) designed for the teaching laboratory. Frontiers in Plant Science 3: 118. doi.org/10.3389/fpls.2012.00118

Wendell, D.L. and Pickard, D. (2007). Teaching human genetics with mustard: rapid cycling Brassica rapa (Fast Plants type) as a model for human genetics in the classroom laboratory. CBE-Life Sciences Education 6: 179-185. doi.org/10.1187/cbe.07-02-0010